Aktuelle Masterarbeitsthemen der Forschungsgruppe Pharmazeutische Biologie
Current Master's Theses-Projects of the Research Group in Pharmaceutical Biology

 

Forschungsgebiet
Research Field
Kontakt Betreuer/in
Contact Supervisor
MSc PharmazieMSc Drug SciencesBemerkungen
Comments

Identifying natural products inhibiting aberrant proliferation signaling in melanoma

The incidence of melanoma, the most fatal dermatological cancer, is dramatically rising. In more than 50% of malignant melanomas the BRAF V600E mutation is present, leading to aberrant MAPK signaling and uncontrolled proliferation. Vemurafenib, a specific inhibitor of the V600E mutated form of B-Raf, has been approved in 2011 for the treatment of metastatic melanomas. Despite spectacular initial results, patients treated with vemurafenib relapse within 2-18 months due to drug resistance. Novel inhibitors targeting aberrant proliferation signaling (MAPK and PI3K pathways) in melanoma are therefore urgently needed.

A total of 2576 crude extracts were screened in our lab using a high-content screening (HCS) assay that quantifies MAPK/PI3 signaling in melanoma cells, and a total of 66 extracts were identified as active hits. The activity-profiling of these hits (Master Thesis project in 2019) allowed us to correlate the activity with compounds in the crude extracts. Based on this work, extracts were prioritized for scale-up extraction and isolation.

The aim of this project is now to isolate active compounds and to identify their structures. You will be integrated into a team of 2 PhD students working on this project. You will learn and use HPLC-MS (analytical method development), various chromatography techniques for the isolation of natural products, and NMR for structure elucidation of isolated compounds.

Keywords: Melanoma, isolation of bioactive natural products, structure elucidation

Dr. Eliane Garojaja
No longer available
Identifying Natural products inhibiting the mTOR Complex1 (mTORC1)
Dr. Eliane Garojaja

No longer available
Molecular contraception or infertility treatment? Isolation of molecules from plants interacting with ABHD2, an enzyme involved in male fertility

Dr. Ombeline Dantonjaja

No longer available
Phytochemical investigation of Daphne extracts and daphnetoxin, promising substances for cosmetics and dermatology

Prof. Dr. Olivier Potteratjaja

No longer available
Investigation of the chemical composition of the historical remedy Theriak

Prof. Dr. Olivier Potteratjanein

Entwicklung galenischer und analytischer Grundlagen für die magistrale Zubereitung einer Salbe mit einem lipophilen Extrakt aus Viscum album

Ziele:

  • Entwicklung der Herstellung eines Wirkstoff-Konzentrats (Halbfabrikat) aus einem neuen pflanzlichen Wirkstoff (lipophiles Vielstoffgemisch aus Viscum album); Auswahl einer geeigneten Grundlage.
  • Für die magistralen Zubereitung der Salbe: Auswahl einer geeigneten, üblichen Salbengrundlage, in welche das Wirkstoff-Konzentrat eingearbeitet werden kann.
  • Entwicklung einer Identitätsprüfung des Wirkstoff-Konzentrats zur Durchführung der Eingangskontrolle in der Apotheke (rechtliche Bedingungen in DE, CH): Hierfür kommt insbesondere die Dünnschichtchromatographie (Fingerprint) zum Einsatz. Konkret geht es um die Evaluierung
    - von geeigneten DC-Systemen und Referenzsubstanzen, unter Berücksichtigung  bestehender Pharmakopöe-Methoden,
    - der praktischen Umsetzbarkeit mit der Ausrüstung im Labor einer Apotheke – im Vergleich zur Durchführung mit automatisierten Geräten.
  • Erstellung von Anleitungen für die Herstellung und Prüfung in der Apotheke
Dr. Stephan BaumgartnerjaArbeitsort: Arlesheim/Verein für Krebsforschung

Neurodegeneration; aging; stress; mitochondria; neuronal cell culture; drugs targeting mitochondria; plant extracts

Prof. Anne Eckertjaja
Dissolution tests of inhalation drugs; correlating dissolution and permeability behavior of inhalation drugs to its local pulmonary pharmacokinetics; integration of in vitro tests, such as dissolution and permeability assessments into pharmacokinetic models.Prof. Günther Hochhausjaja
Generation of cell lines, stably expressing ion channels; assay development and validation; drug screenin; cell culture; electrophysiology; fluorescenc based methodsDr. Simon Hebeisenjaja
Poorly water-soluble active ingredients, lipid-containing self-emulsifying systems, solid dosage formsProf. Martin Kuentzjaja
Development of planar chromatographic methods for analysis of medicinal plants and other applicationsDr. Eike Reichjaja
Lipid-based solid dispersions; Analysis; Diffusing Wave SpectroscpoyProf. Martin Kuentzjaja
Ionic Liquids; Formulation of hydrophobic drugs; Polymers; Solid dosage formsProf. Martin Kuentzjaja